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Test
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Method
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PRO-169
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|---|
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Quality
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Appearance
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Slightly yellow, opalescent liquid
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pH
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6.2
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Charge of heterogeneity
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CEX-HPLC
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55.1% main peak, 34.3% acidic variants, and 10.7 basic variants
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Structure
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Intact mass LC/MS, G0F/G0F
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149201 Da
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Deglycosylated partially reduced LC/MS
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Light chain mass = 23450.8 Da, deglycosylated heavy chain mass = 49717.5 Da
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Oligosaccharide profile
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N-Glycan profiling
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79.2% G0, 16.1% G1, and 0.9% G2
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Other
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SPR
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KD = 6.54 × 10−6
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Vitreous Pharmacokinetics
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SPR
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CMax ± SD = 593.7 ± 42.6 µg/ml
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TMax ± SD = 0.53 ± 0.8 days
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T1/2 ± SD = 4.99 ± 0.9 days
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- CEX-HPLC: cation exchange chromatography-high performance liquid chromatography; CMax: peak maximum concentration; KD: binding constant; LC/MS: liquid chromatography/mass spectrometry; SD: standard deviation; SPR: surface plasmon resonance; T1/2: half-life time; TMax: time to maximal concentration
